Developmental events associated with the critical stage for sex determination in wild-rice florets

The developmental events of florets and a critical stage for sex determination in two wild‐rice populations (Zizania palustriscv. Franklin andZizania palustriscv. K‐2Pi) have been identified. Formation of bisexual florets precedes the development of both male and female florets. Developmental indicators, established by measuring the length of florets and panicles, indicate that the critical stage for sex determination occurs when floret and panicle lengths are 1–2 mm and 3 cm, respectively. The stage of floret development at which sex determination occurs is the same in the two investigated wild‐rice populations. Organ suppression in bisexual florets is an essential step for sex determination during the formation of unisexual florets. Histological examination of suppressed stamens or pistils in unisexual florets of wild‐rice indicates that cell death does not occur during sex determination. In addition, the length of anthers and pistils in bisexual florets indicates that floral development in the transition zone is normal when compared with the male florets in the male spikelets and female florets in the female spikelets.