Fabrication and characterization of nano-particle biomaterial scaffold for treating burn wounds

Healing burn wounds is a complex process and challenging to design an ideal scaffold for tissue regeneration. The inflammatory phase is critical in the healing of burn wounds due to the presence of bacteria and/or endotoxins. This research deals with the fabrication and development of electrospun metal oxide nanoparticle incorporated polyvinyl alcohol nanofiber in the healing of second-degree burn wounds. Structural and Physico-chemical characterization of the scaffold was performed. Structural analysis is performed to confirm the incorporation of the Molybdenum nanoparticle into the PVA scaffold. The biocompatibility and cellular adhesion of the fabricated scaffold are analyzed quantitatively by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay and crystal violet assay, respectively. Antimicrobial activity is tested for PVA scaffold against both gram-positive and gram-negative bacteria. Our results, therefore, open up a new avenue for designing a nanoparticle incorporated delivery system in wound therapeutics, especially for second-degree burns. Graphical abstract