Isolation and identification of some bacterial species causing urinary tract infections in Holy Karbala Iraq

Objectives: Some bacterial Species Causing Urinary Tract Infections were opportunistic pathogenic nosocomial associated with the hospital settings infections such as emphysematous cystitis, phylonephritis, renal or perinephric abscess, bacteremia and renal papillary necrosis occur more commonly in diabetic patients. the need to identify the spread of these bacteria and their resistance to the antibiotics which are used in Iraqi hospitals. Methods: A total of 55 clinical samples which include (25 urine samples from Non - Diabetic patients and 20 samples from diabetic patients) and 10 catheter Samples. samples were collected from AL-Hussein Medical City in Karbala during the period from July to October 2020 Furthermore, Polymerase Chain Reaction Technique (PCR) was performed to identify phoA gene in Escherichia coli. Results: 14 isolates of Some bacterial Species Causing Urinary Tract Infections have been identified. Susceptibility of 14 bacteria isolates Causing Urinary Tract Infections to 15 different antibacterial has been evaluated, the results showed that the PCR technique was detected the specific phoA gene in all E. coli isolates.