Komagataeibacter saccharivorans strain BC-G1: an alternative strain for production of bacterial cellulose

The current study represents the isolation and characterization of cellulose producing bacteria from rotten grape fruit. The best Bacterial Cellulose producer isolated from rotten grape fruit was identified as Komagataeibacter saccharivorans BC-G1 upon biochemical and molecular characterization. Bacterial Cellulose production was done in Hestrin and Schramm (HS) medium under static condition. Studies showed that BC was produced as a thick layer on the surface of medium and yield of BC was 14 g/L after 96 h of fermentation period. BC films were characterized by Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy and X-ray Diffraction. SEM analysis revealed the presence of highly extended interwoven cellulose fibrils. Characteristics of FTIR spectrum depicted the presence of characteristic absorption bands and less crystalline behavior of BC. XRD patterns demonstrated three major characteristic diffraction peaks corresponding to (110), (100) and (002) indicating presence of less crystalline behavior of BC produced in this study. Results confirmed the produced biopolymer by K. saccharivorans BC-G1 is BC.