Banana peel metalloprotease characterizations

Proteases are a group of enzymes that cleaves on peptide bonds specifically. Proteolytic enzymes are of global importance because of their widespread applications in therapeutics and commercial purposes. Despite existing proteases, researchers are focusing in finding some cost effective protease source to meet out the demand in the enzyme market. Amongst the various sources available, nendran banana peel had been identified as a potential source of metalloprotease. The isolated biomolecules could be immobilized into various physical forms and has extensive potential in diverse fields. The characterization of the isolated enzyme was analyzed using particle size analyzer, TEM, TGA, DSC, MALDI-TOF and proton NMR. Based on the physical parameters techniques the right vehicle could be finalized. The average particle size of the molecules was found to be 2110 d.nm with a poly dispersity index of 0.299 and the intercept is 0.860. The zeta potential was found to be -8.93 mV with a zeta deviation of 15.6 mV and conductivity of 4.03 (mS/cm). From the results of TEM it is well clear that there are no signs of agglomeration among the particles. With the help of TGA and DSC studies it is well understood that the proteolytic enzymes have the utmost thermal stability. The results suggest that the enzyme could add a new insight on protease research.