Increased Efficiency of Radiation Inactivation of Virions by Gold Nanoparticles

Gold nanoparticles (GNPs) are promising radiosensitizers for cancer radiotherapy. Moreover, they can be used in the same way for radiation processing and sterilization. Such application of GNPs is of practical interest since it may significantly reduce the dose load and expand the application of radiation treatment. In the present study, the high radiosensitization effect of GNPs in relation to viral particles is demonstrated for the first time. The preparations of tobacco mosaic virus (TMV) are used as an experimental model, insofar as this virus has the same properties as animal and human ones but is safe for humans. Irradiation with 45 kVp X‐ray to the doses of 4 and 7 kGy leads to a decrease in the infectious activity of TMV virions up to 1.92‐ and 2.70‐fold, respectively. At the same time, irradiation in the presence of 0.4 mg mL−1 of 12 nm spherical GNPs increases the efficiency of virus inactivation up to 15‐ and 22‐fold. The GNPs enhance both the damage to capsid protein due to the enhanced generation of reactive oxygen species and genome RNA due to the emission of secondary radiation. These results show the great prospects of the application of high‐Z nanoparticles in radiation treatment. The high radiosensitization effect of gold nanoparticles (GNPs) in relation to viral particles is demonstrated for the first time on the example of tobacco mosaic virus (TMV) model. In the presence of 12 nm GNPs the efficiency of X‐ray irradiation of TMV preparations is increased up to 15‐fold at 4 kGy and up to 22‐fold at 7 kGy.