Effects and mechanism of Hcy on apoptosis and proliferation of cardiomyocytes

Effects and mechanism of Hcy on apoptosis and proliferation of cardiomyocytes

Objective　To investigate the effect and mechanism of homocysteine on apoptosis and proliferation of cardiomyocytes. Methods　Cardiomyocytes were stimulated with homocysteine at the concentration of 3 mmol/L for 24 h. Cells were divided into control group and homocysteine group (Hcy group). Western bl...

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Veröffentlicht in:Jie fang jun yi xue za zhi 2022-01, Vol.47 (10), p.984
Hauptverfasser: Da-Yue, LIU, Xiao-Yan, DONG, Ling-Bo, XU, Hui-Ping, ZHANG, Shang-Kun QUAN, Sheng-Chao, MA, Yong-Sheng, BAI, YANG, Yong, Gui-Zhong, LI
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Apoptosis
Cardiomyocytes
Flow cytometry
Homocysteine
Proteins
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container_issue 10
container_start_page 984
container_title Jie fang jun yi xue za zhi
container_volume 47
creator Da-Yue, LIU
Xiao-Yan, DONG
Ling-Bo, XU
Hui-Ping, ZHANG
Shang-Kun QUAN
Sheng-Chao, MA
Yong-Sheng, BAI
YANG, Yong
Gui-Zhong, LI
description Objective　To investigate the effect and mechanism of homocysteine on apoptosis and proliferation of cardiomyocytes. Methods　Cardiomyocytes were stimulated with homocysteine at the concentration of 3 mmol/L for 24 h. Cells were divided into control group and homocysteine group (Hcy group). Western blotting was detected the expression of TLR4, Myeloid differentiation factor 88 (MyD88), NF-κB, p-NF-κB, the expression of apoptosis related proteins Bax, cleaved-caspase-3 and anti-apoptotic protein Bcl-2; Flow cytometry was detected the apoptosis rate of cardiomyocyte, EdU staining was observed the proliferation of cardiomyocytes. After TLR4 interference fragment was transfected into cardiomyocytes, the interference fragment was verified by Western blotting. The cells were transfected with si-NC or si-TLR4 and treated with Hcy. Western blotting was detected the expression of MyD88, NF-κB and p-NF-κB，the expression of apoptosis related protein and anti-apoptosis protein in cardiomyocytes; The apoptosis rate of cardi
doi_str_mv 10.11855/j.issn.0577-7402.2022.10.0984
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subjects Apoptosis
Cardiomyocytes
Flow cytometry
Homocysteine
Proteins
title Effects and mechanism of Hcy on apoptosis and proliferation of cardiomyocytes
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