Effects and mechanism of Hcy on apoptosis and proliferation of cardiomyocytes

Objective To investigate the effect and mechanism of homocysteine on apoptosis and proliferation of cardiomyocytes. Methods Cardiomyocytes were stimulated with homocysteine at the concentration of 3 mmol/L for 24 h. Cells were divided into control group and homocysteine group (Hcy group). Western blotting was detected the expression of TLR4, Myeloid differentiation factor 88 (MyD88), NF-κB, p-NF-κB, the expression of apoptosis related proteins Bax, cleaved-caspase-3 and anti-apoptotic protein Bcl-2; Flow cytometry was detected the apoptosis rate of cardiomyocyte, EdU staining was observed the proliferation of cardiomyocytes. After TLR4 interference fragment was transfected into cardiomyocytes, the interference fragment was verified by Western blotting. The cells were transfected with si-NC or si-TLR4 and treated with Hcy. Western blotting was detected the expression of MyD88, NF-κB and p-NF-κB，the expression of apoptosis related protein and anti-apoptosis protein in cardiomyocytes; The apoptosis rate of cardi