Carriage of CdtB Encoding Campylobacter spp., Salmonella enterica, and Yersinia entercolitica in Patients with Gastroenteritis and Irritable Bowel Syndrome
Introduction Cytolethal distending toxin (Cdt) is one of the bacterial toxins that present in a variety of gram-negative human pathogens, such as E. coli, Salmonella spp., and Campylobacter spp. CDT is composed of three subunits encoded by three adjacent genes, including cdtA , cdt B, and cdt C. cdt...
Gespeichert in:
Veröffentlicht in: | Digestive diseases and sciences 2022-12, Vol.67 (12), p.5522-5528 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Introduction
Cytolethal distending toxin (Cdt) is one of the bacterial toxins that present in a variety of gram-negative human pathogens, such as
E. coli, Salmonella
spp., and
Campylobacter
spp. CDT is composed of three subunits encoded by three adjacent genes, including
cdtA
,
cdt
B, and
cdt
C.
cdtB
has been shown to have toxic activity and cause DNA damage in host cells. Despite its presence in different bacterial species, the role of CdtB in acute and chronic infections, such as gastroenteritis and irritable bowel syndrome (IBS), is unclear. To analyze this correlation, we studied the prevalence of
cdt
B among different enteropathogenic bacteria in patients with gastroenteritis and IBS compared with healthy people.
Materials and Methods
In this cross-sectional descriptive study, 230 stool samples were collected from patients with gastroenteritis, IBS, and healthy people. The presence of CdtB encoding bacteria, including
Escherichia coli
,
Campylobacter
spp.,
Yersinia entercolitica
,
Providencia alkalifacience
, and
Salmonella enterica
, was examined by polymerase chain reaction using genus-specific primers.
Results
Out of 230 stool samples, CdtB encoding
Campylobacter
spp. were found in 34.6% (52/150), 6.25% (5/80), and 4% (2/50) of the patients with gastroenteritis, IBS, and the control group, respectively. Carriage of CdtB encoding
Salmonella enterica
was characterized among 5.3% (8/150) of the patients with gastroenteritis and 17.5% (14/80) of the IBS patients. Although none of the patients carried CdtB encoding
E. coli
and
Providencia
spp.,
cdt
B of
Y. enterocolitica
was detected in one of the patients with gastroenteritis (0.6%). Statistical analysis showed significant correlation between infection with CdtB encoding
Campylobacter
spp. and IBS-D subtype. No significant correlation was found between infection with CdtB encoding bacteria and other clinical and demographic data.
Conclusion
Our results confirmed a relatively higher frequency of CdtB encoding bacteria in the intestine of patients with gastroenteritis and those with IBS compared with healthy individuals. Regarding the frequency of CdtB encoding
Salmonella
and
Campylobacter
bacteria, it was proposed that infection with these enteropathogens could be considered a risk factor for the development or progression of IBS among Iranian patients. Further studies are needed to establish this involvement. |
---|---|
ISSN: | 0163-2116 1573-2568 |
DOI: | 10.1007/s10620-022-07468-x |