c-Fos Expression in Rat Medulla Oblongata after Subdiaphragmatic Vagotomy and Various Antigens Administration

Neuroimmune interactions pathways are the current interests of neuroimmunophysiology. As it shown, n. vagus is a part of neuroimmune communications, so determination of activation patterns of n. vagus is the relevant task. The aim of the study is to determine the amount of с-Fos-positive neurons in nuclei tractus solitarius (NTS) and dorsal motor nuclei (DMX) in rat medulla oblongata after subdiaphragmatic vagotomy and various antigens administration. Immunohistochemistry and morphometry were performed for determination of с-Fos-positive neurons amount in different zones of NTS and DMN. In the cells of DMX were not abserved any с-Fos-positive neurons in control and after subdiaphragmatic vagotomy. LPS administration (500 µg/kg) induced the increasing of с-Fos-positive neurons amount in NTS, particularly in medial NTS. Subdiaphragmatic vagotomy leads to increasing of с-Fos-positive neurons amount in NTS after LPS or SEB (500 µg/kg) administration. LPS injection after subdiaphragmatic vagotomy induced an activation of neurons of the lateral, medial and central NTS, while SEB administration induced only medial NTS activation. Therefore, the pattern of NTS response to the introduction of certain antigens are shown. Subdiaphragmatic vagotomy leads to activation of NTS after the introduction of LPS and SEB.