Preliminary study on the improvement of identifying effect of Tc-99 m labeled C595 monoclonal antibody in MUC1 imaging of ovarian cancer

MUC1 imaging helps to guide the corresponding biological therapy of ovarian cancer. Herein, C595 antibody was cleaved to fab fragment, and then coupled with NHS-DTPA. The imaging effect using 99m Tc-C595-Fab was evaluated on OVCAR-3 cells derived xenograft models. In results, the cleaved antibody did not affect the Tc-99 m labeling effect and in vitro stability. SPECT imaging proved the decreased accumulation of fragment in liver, together with the improved targeting efficiency in tumor, realizing the faster tumor targeting and higher tumor/liver ratio. Conclusively, C595 fragment realized the more efficient SPECT imaging of ovarian cancer.