Synthesis, characterization and cytotoxicity activity of new compounds derived from adenine

Four new compounds have been successfully synthesized from adenine ring by its reaction with chloroacetyl chloride to produce 1-(6-amino-9H-purin-9-yl)-2-chloroethan-1-one (1) which is used to synthesize 1-(6-amino-9H-purin-9-yl)-2-hydrazinylethan-1-one (2) via the reaction with hydrazine hydrate and the last compound used as precursor for two new Schiff bases(1-(6-amino-9H-purin-9-yl)-2-(2-(4 dimethylamino)benzylidene)hydrazinyl)ethan-1-one (3) and 1-(6-amino-9H-purin-9-yl)-2-(2-(4-chlorobenzylidene)hydrazinyl)ethan-1-one (4) through reaction with 4-(dimethylamino)benzaldehyde and 4-chloro benzaldehyde. The new compounds were characterized by some spectroscopic techniques such as (FT-IR, 1HNMR and APT 13CNMR). The biological activity of synthesized compounds was tested for their cytotoxicity activity against breast cancer cell line type HepG2 and SK-GT2 with different concentrations. The biological results shown these compounds have promising cytotoxicity activity against HepG2 and SK-GT2 cell line.