Effect of sodium chloride on Acinetobacter baumannii growth and Lipopolysaccharide components

Acinetobacter baumannii is a major opportunistic nosocomial pathogen. Sodium chloride is one of the elements that necessary for the survival of some bacterial species. The purpose of this study is to identify the effect of NaCl on A. baumannii . In this study two isolates of A. baumannii have been used, the local isolate HHR1 and the global isolate ATCC 17904. Firstly, the influence of NaCl on the growth of two isolates of A. baumannii was monitored and the effect of two concentrations of NaCl on Lipopolysaccharide components was determined by SDS-PAGE. The result showed that HHR1 after 20 hr of incubation period was reached to 0.6 at OD600 in the presence of salt, while the control was continued, whereas, and A. baumannii ATCC didn’t exert any defect growth. Moreover, NaCl has greatly affected the LPS components, where only O-antigen has been appeared under 1% NaCl in A. baumannii HHR1 and 3% NaCl in A. baumannii ATCC.