Translating solution to solid phase glycosylation conditions

Optimization of glycosylation conditions for automated glycan assembly is highly challenging, demands wasteful use of precious building blocks, and relies on nontrivial analyses. We developed a semi-quantitative method for automated optimization of glycosylation temperature that utilized minute quantities of donors and translated those conditions to solid-phase glycan assembly. Fast, economical and automated strategy for optimizing glycosylation temperature was developed and utilized for mirroring solution to solid-phase conditions.