A guide to genetically encoded tools for the study of H2O2

Cell metabolism heavily relies on the redox reactions that inevitably generate reactive oxygen species (ROS). It is now well established that ROS fluctuations near basal levels coordinate numerous physiological processes in living organisms, thus exhibiting regulatory functions. Hydrogen peroxide, the most long‐lived ROS, is a key contributor to ROS‐dependent signal transduction in the cell. H2O2 is known to impact various targets in the cell; therefore, the question of how H2O2 modulates physiological processes in a highly specific manner is central in redox biology. To resolve this question, novel genetic tools have recently been created for detecting H2O2 and emulating its generation in living organisms with unmatched spatiotemporal resolution. Here, we review H2O2‐sensitive genetically encoded fluorescent sensors and opto‐ and chemogenetic tools for controlled H2O2 generation. Hydrogen peroxide (H2O2) is one of the most important signaling molecules, which coordinates many physiological processes ranging from development to wound healing. Identification of mechanisms underlying specificity of H2O2‐dependent signal transduction is a central issue in redox biology. A palette of genetically encoded tools for generation and detection of H2O2 with unprecedented spatiotemporal resolution has been recently created to resolve this issue. Here, we present an overview of these tools.