Adsorbents with a Covalently Bonded Polymer Layer for Hydrophilic Interaction Liquid Chromatography

Stationary phases based on silica modified with polyvinylpyridine and polyethyleneimine of different structures using 1,4-butanediol diglycidyl ether as a spacer are obtained. The study and comparison of the chromatographic properties of sorbents modified with polyamines and polyethylene glycol are performed using the Tanaka test for hydrophilic stationary phases and via the separation of model mixtures of sugars, amino acids, and water-soluble vitamins. It is shown that the nature and structure of the polymer in the functional layer affect both the selectivity and efficiency for polar compounds in hydrophilic interaction liquid chromatography mode.