Study of the potexvirus ribonucleoproteins signal of assembly

Formation of viral ribonucleoprotein particles during incubation of the Alternanthera mosaic virus 5’-end transcripts with Potato virus X (members of the Potexvirus genus) coat protein was studied. It was shown that the capping of Alternanthera mosaic virus transcripts affects the efficiency of their interaction with a coat protein. At the same time, the removal of the cap structure from previously capped Alternanthera mosaic virus transcripts did not prevent assembly of viral ribonucleoproteins. The removal of the first 100 nucleotides (the likely site for assembly initiation) had no effect on the formation of viral ribonucleoproteins. The additional evidences that the potexvirus ribonucleoproteins assembly does not depend on the RNA nucleotide sequence were obtained.