Study on Toxicity of 9 Biocontrol Microbial Products to Adult Population of Tobacco Beetle, Lasioderrma serricorne (Fabricius)

[Conclusions] According to the three stages of tube rubbing, it is found that the control effects of Lujinwa B. thuringiensis powder and Meichongzhi B. bassiana powder are the best, and the results will provide technical support for the biological control of L. serricorne adults. [...]Bacillus thuringiensis has good infection and lethal effect on L. serriicorne. At present, although many biocontrol bacteria have been found to have good infection and lethal effect on L. serricorne adults, there are no biocontrol products widely used in the field of L. serricome adults control, and the comparative study of related products in the control of L. serricorne adults outdoors and indoors i s still blank. [...]in this study, 9 biocontrol microbes were used as test materials to study their toxicity to the experimental population of L. serricorne adults, in order to provide technical support for the biological control of L. serricome adults. 2Materials and methods 2.1 Insects collection and breeding Tobacco beetle (L. serricorne) adults were collected from Guiyang Redrying Factory, Guizhou Tobacco Redrying Co., Ltd. , and were reared and purified for at least 5 generations in the laboratory of Guizhou Engineering Research Center for Mountain Featured Fruits and Products of Guizhou Light Industry Technical College. 200 g of feed was composed of corn, smoke foam and brewer's yeast at the ratio of 9: 5 : 5 , and L. serricorne were grown under the conditions of temperature (33 ± 1)°C, relative humidity (75 ± 5) % , and light 10 D: 14 L. 2. 2.4 Data analysis Mortality rate ( % ) = ( Number of dead insects/Number of test insects) x100; Corrected mortality rate (%) = ( Mortality rate in experimental group - Mortality rate in control group) / (1- Mortality rate in control group) x 100.