Evaluation of Anti-Inflammatory Activity of Metronidazole Treatment On Carrageenan Induced Paw Edema in Mice

Besides its conventional use, it has been started to use both topically and orally in different indications such as acne vulgaris, Crohn's disease, periorificial dermatitis, rosacea and seborrheic dermatitis (Boeck, Abeck, Werfel, & Ring, 1997; Nishimuta & Ito, 2003; Pradhan, Madke, Kabra, & Singh, 2016; Seckin, Gurbuz, & Akin, 2007; Zip, 2010). Furthermore, the functional role of metronidazole on acute inflammation is not known yet. [...]in our study we aimed to induce inflammation acutely in mice by using the carrageenan to investigate the acute and long-time anti-inflammatory effect of metronidazole at increasing doses. Change in paw thickness (A)=x- x0 Edema inhibition (%) = [(Control A - Treatment A) / Control A] x 100 Potency was calculated according to the formula below. Because edema occurred mostly 2 hours after the carrageenan injection, the edema inhibition % of the 2nd hour was selected for the potency calculations. RESULTS Carrageenan-induced paw edema in the groups administered with 2, 20, and 200 mg/kg metronidazole was reduced at all measurement time points compared to the control group (Figure 1.)