Acute toxicity of soybean extract with targeted Lunasin (ET-Lun)

ET-Lun is an extract containing Lunasin as an active compound. Lunasin was extracted using PBS with pH 7,4 from defatted soybean powder. Several studies proved ET-Lun could reduce the expression of COX-2 and iNOS. ET-Lun can inhibit angiogenesis, increase apoptosis and reduce dysplasia. ET-Lun might also decrease EGFR expression in DMBA induced breast cancer rats. This study aimed to evaluate the acute toxicity of ET-Lun using Sprague Dawley (SD) rats. Two groups (n=10) were orally given a single dose of ET-Lun at 2000 mg/kg and 5000 mg/kg weight. The control group (n = 5) received only vehicle distilled water. In the end, the rats were sacrificed. The blood was collected for haematological evaluation and liver, and kidney histopathology was examined afterwards. There were no toxic signs on the administration of ET-lun doses of 2000 and 5000 mg/kg. The histopathology of the liver and kidney groups showed no difference between the treatment group and the control group. Furthermore, creatinine, urea, and AST and ALT levels showed no difference between the treatment group and control (p > 0.05). ET-Lun has LD50 more than 5000 mg/kg BW and is practically non-toxic.