QTL mapping for salt tolerance in wheat line CH7034

The identification of wheat salt-tolerant germplasm is of great significance for making full use of saline land and ensuring food security. CH7034 is a wheat salt-tolerant line bred by our laboratory. In order to clarify the genetic rules and control loci for salt tolerance, QTL analysis was conducted using a recombinant inbred lines population crossed by CH7034 and salt-sensitive variety SY95-71. Based on the SNP microarray data and salt injury index, six QTL were detected on chromosomes 2A, 2D, 4B, and 5A, including QSI.sxau_2A, QSI.sxau_2D, QSI.sxau_4B.1, QSI.sxau_4B.2, QSI.sxau_5A.1, and QSI.sxau_5A.2. Among them, QSI.sxau_5A.1 was detected in all three salt stress experiments and had the highest phenotypic variance explained (15.73%-20.18%), which was different from other salt-tolerance loci reported on chromosome 5AL. Seven SSR markers were developed and integrated in the QSI.sxau_5A.1 interval, and the peak of LOD was further determined at SSR-D1. Based on the transcriptome database, 12 high-confidence