Effects of nanoaggregation on isoindigo-based fluorophores for near-infrared bioimaging applications
In this work, we have taken a donor-acceptor-donor (D-A-D) fluorophore ( II-EDOT-TPA ) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative ( PEG-II-EDOT-TPA ) was synthesized. The self-assembly and colloidal properties of both nanoaggreg...
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| Veröffentlicht in: | Molecular systems design & engineering 2022-08, Vol.7 (8), p.96-914 |
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| creator | Sparks, Nicholas E Dorris, Austin Vijayan, Sajith M Chandrasiri, Indika Zia, Mohammad Farid Flynt, Alex Hammer, Nathan I Watkins, Davita L |
| description | In this work, we have taken a donor-acceptor-donor (D-A-D) fluorophore (
II-EDOT-TPA
) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative (
PEG-II-EDOT-TPA
) was synthesized. The self-assembly and colloidal properties of both nanoaggregates were comparatively assessed. Photophysical and morphological characterization of the LDBC encapsulated
II-EDOT-TPA
and
PEG-II-EDOT-TPA
nanoaggregates was performed, which showed the photophysical and morphological properties differed greatly when comparing the two. Both nanoaggregate types were incubated with HEK-293 cells in order to measure cell viability and perform confocal fluorescence microscopy. Minimal cytotoxicity values ( |
| doi_str_mv | 10.1039/d2me00014h |
| format | Article |
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II-EDOT-TPA
) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative (
PEG-II-EDOT-TPA
) was synthesized. The self-assembly and colloidal properties of both nanoaggregates were comparatively assessed. Photophysical and morphological characterization of the LDBC encapsulated
II-EDOT-TPA
and
PEG-II-EDOT-TPA
nanoaggregates was performed, which showed the photophysical and morphological properties differed greatly when comparing the two. Both nanoaggregate types were incubated with HEK-293 cells in order to measure cell viability and perform confocal fluorescence microscopy. Minimal cytotoxicity values (<20%) were seen with the two nanoaggregate forms, while both types of nanoaggregates were found to accumulate into the lysosomes of the HEK-293 cells. This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.
This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.</description><identifier>ISSN: 2058-9689</identifier><identifier>EISSN: 2058-9689</identifier><identifier>DOI: 10.1039/d2me00014h</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Block copolymers ; Chemical compounds ; Encapsulation ; Lysosomes ; Medical imaging ; Polyethylene glycol ; Self-assembly ; Toxicity</subject><ispartof>Molecular systems design & engineering, 2022-08, Vol.7 (8), p.96-914</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-d5b5373b0a682c507f9bf02085a8bc5f993b4b4ed2cae4f6cae5f5c428eba1953</citedby><cites>FETCH-LOGICAL-c281t-d5b5373b0a682c507f9bf02085a8bc5f993b4b4ed2cae4f6cae5f5c428eba1953</cites><orcidid>0000-0002-8925-8045 ; 0000-0002-6221-2709 ; 0000-0002-2373-3544 ; 0000-0002-0943-7220 ; 0000-0002-4075-4435</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sparks, Nicholas E</creatorcontrib><creatorcontrib>Dorris, Austin</creatorcontrib><creatorcontrib>Vijayan, Sajith M</creatorcontrib><creatorcontrib>Chandrasiri, Indika</creatorcontrib><creatorcontrib>Zia, Mohammad Farid</creatorcontrib><creatorcontrib>Flynt, Alex</creatorcontrib><creatorcontrib>Hammer, Nathan I</creatorcontrib><creatorcontrib>Watkins, Davita L</creatorcontrib><title>Effects of nanoaggregation on isoindigo-based fluorophores for near-infrared bioimaging applications</title><title>Molecular systems design & engineering</title><description>In this work, we have taken a donor-acceptor-donor (D-A-D) fluorophore (
II-EDOT-TPA
) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative (
PEG-II-EDOT-TPA
) was synthesized. The self-assembly and colloidal properties of both nanoaggregates were comparatively assessed. Photophysical and morphological characterization of the LDBC encapsulated
II-EDOT-TPA
and
PEG-II-EDOT-TPA
nanoaggregates was performed, which showed the photophysical and morphological properties differed greatly when comparing the two. Both nanoaggregate types were incubated with HEK-293 cells in order to measure cell viability and perform confocal fluorescence microscopy. Minimal cytotoxicity values (<20%) were seen with the two nanoaggregate forms, while both types of nanoaggregates were found to accumulate into the lysosomes of the HEK-293 cells. This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.
This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.</description><subject>Block copolymers</subject><subject>Chemical compounds</subject><subject>Encapsulation</subject><subject>Lysosomes</subject><subject>Medical imaging</subject><subject>Polyethylene glycol</subject><subject>Self-assembly</subject><subject>Toxicity</subject><issn>2058-9689</issn><issn>2058-9689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpNkEFLAzEQhYMoWGov3oWAN2E1yW7S5Ci1tULFi56XJJtsU9pknWwP_nu3ragwzAzMx3vDQ-iakntKSvXQsJ0jhNBqfYZGjHBZKCHV-b_9Ek1y3hwYIQXjYoSauffO9hknj6OOSbctuFb3IUU8VMgpxCa0qTA6uwb77T5B6tYJXMY-AY5OQxGiBw3D2YQUdroNscW667bBHoXyFbrwepvd5GeO0cdi_j5bFqu355fZ46qwTNK-aLjh5bQ0RAvJLCdTr4wnjEiupbHcK1WaylSuYVa7youhc89txaQzmipejtHtSbeD9Ll3ua83aQ9xsKyZUEIxzuiBujtRFlLO4HzdwfA1fNWU1Icg6yf2Oj8GuRzgmxMM2f5yf0GX3yQucaY</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Sparks, Nicholas E</creator><creator>Dorris, Austin</creator><creator>Vijayan, Sajith M</creator><creator>Chandrasiri, Indika</creator><creator>Zia, Mohammad Farid</creator><creator>Flynt, Alex</creator><creator>Hammer, Nathan I</creator><creator>Watkins, Davita L</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0002-8925-8045</orcidid><orcidid>https://orcid.org/0000-0002-6221-2709</orcidid><orcidid>https://orcid.org/0000-0002-2373-3544</orcidid><orcidid>https://orcid.org/0000-0002-0943-7220</orcidid><orcidid>https://orcid.org/0000-0002-4075-4435</orcidid></search><sort><creationdate>20220801</creationdate><title>Effects of nanoaggregation on isoindigo-based fluorophores for near-infrared bioimaging applications</title><author>Sparks, Nicholas E ; Dorris, Austin ; Vijayan, Sajith M ; Chandrasiri, Indika ; Zia, Mohammad Farid ; Flynt, Alex ; Hammer, Nathan I ; Watkins, Davita L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-d5b5373b0a682c507f9bf02085a8bc5f993b4b4ed2cae4f6cae5f5c428eba1953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Block copolymers</topic><topic>Chemical compounds</topic><topic>Encapsulation</topic><topic>Lysosomes</topic><topic>Medical imaging</topic><topic>Polyethylene glycol</topic><topic>Self-assembly</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sparks, Nicholas E</creatorcontrib><creatorcontrib>Dorris, Austin</creatorcontrib><creatorcontrib>Vijayan, Sajith M</creatorcontrib><creatorcontrib>Chandrasiri, Indika</creatorcontrib><creatorcontrib>Zia, Mohammad Farid</creatorcontrib><creatorcontrib>Flynt, Alex</creatorcontrib><creatorcontrib>Hammer, Nathan I</creatorcontrib><creatorcontrib>Watkins, Davita L</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Molecular systems design & engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sparks, Nicholas E</au><au>Dorris, Austin</au><au>Vijayan, Sajith M</au><au>Chandrasiri, Indika</au><au>Zia, Mohammad Farid</au><au>Flynt, Alex</au><au>Hammer, Nathan I</au><au>Watkins, Davita L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of nanoaggregation on isoindigo-based fluorophores for near-infrared bioimaging applications</atitle><jtitle>Molecular systems design & engineering</jtitle><date>2022-08-01</date><risdate>2022</risdate><volume>7</volume><issue>8</issue><spage>96</spage><epage>914</epage><pages>96-914</pages><issn>2058-9689</issn><eissn>2058-9689</eissn><abstract>In this work, we have taken a donor-acceptor-donor (D-A-D) fluorophore (
II-EDOT-TPA
) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative (
PEG-II-EDOT-TPA
) was synthesized. The self-assembly and colloidal properties of both nanoaggregates were comparatively assessed. Photophysical and morphological characterization of the LDBC encapsulated
II-EDOT-TPA
and
PEG-II-EDOT-TPA
nanoaggregates was performed, which showed the photophysical and morphological properties differed greatly when comparing the two. Both nanoaggregate types were incubated with HEK-293 cells in order to measure cell viability and perform confocal fluorescence microscopy. Minimal cytotoxicity values (<20%) were seen with the two nanoaggregate forms, while both types of nanoaggregates were found to accumulate into the lysosomes of the HEK-293 cells. This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.
This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d2me00014h</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8925-8045</orcidid><orcidid>https://orcid.org/0000-0002-6221-2709</orcidid><orcidid>https://orcid.org/0000-0002-2373-3544</orcidid><orcidid>https://orcid.org/0000-0002-0943-7220</orcidid><orcidid>https://orcid.org/0000-0002-4075-4435</orcidid></addata></record> |
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| ispartof | Molecular systems design & engineering, 2022-08, Vol.7 (8), p.96-914 |
| issn | 2058-9689 2058-9689 |
| language | eng |
| recordid | cdi_proquest_journals_2696925215 |
| source | Royal Society Of Chemistry Journals |
| subjects | Block copolymers Chemical compounds Encapsulation Lysosomes Medical imaging Polyethylene glycol Self-assembly Toxicity |
| title | Effects of nanoaggregation on isoindigo-based fluorophores for near-infrared bioimaging applications |
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