classical live attenuated vaccine for sheep pox

classical live attenuated vaccine for sheep pox

A classical live attenuated sheep pox vaccine was prepared using the Ranipet strain of sheep pox virus (SPV) at the 50th passage in a secondary lamb testicular cell system. The TCID50 and RD50 were 10(9.63)/ml and 10(9.51)/ml. respectively. The SID50 of SPV challenge virus was 10(5)/ml. The vaccine...

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Veröffentlicht in:Tropical animal health and production 2004-05, Vol.36 (4), p.307-320
Hauptverfasser: Bhanuprakash, V, Indrani, B.K, Hedge, R, Kumar, M.M, Moorthy, A.R.S
Format: Artikel
Sprache:eng
Schlagworte:
Animals
antibody formation
Body Temperature
Capripoxvirus - immunology
cell lines
Cell-mediated immunity
dosage
Female
fever
Glucose - metabolism
Guinea Pigs
Humoral immunity
Immunization
Immunization - methods
Immunization - veterinary
Laboratory animals
Laboratory tests
Lamb
lambs
live vaccines
Male
Mice
Neutralization
neutralization tests
Neutralization Tests - veterinary
Poxviridae Infections - immunology
Poxviridae Infections - prevention & control
Poxviridae Infections - veterinary
Poxviridae Infections - virology
Sheep
Sheep Diseases - immunology
Sheep Diseases - prevention & control
Sheep Diseases - virology
Sheeppox virus
Side effects
vaccination
vaccine development
Vaccines
Vaccines, Attenuated - adverse effects
Vaccines, Attenuated - immunology
Vaccines, Attenuated - therapeutic use
Viral Vaccines - adverse effects
Viral Vaccines - immunology
Viral Vaccines - therapeutic use
Viruses
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Beschreibung
Zusammenfassung:A classical live attenuated sheep pox vaccine was prepared using the Ranipet strain of sheep pox virus (SPV) at the 50th passage in a secondary lamb testicular cell system. The TCID50 and RD50 were 10(9.63)/ml and 10(9.51)/ml. respectively. The SID50 of SPV challenge virus was 10(5)/ml. The vaccine was found to have no adverse effects in laboratory animals, and was safe and effective in SPV seronegative lambs. In the field, 660 sheep were vaccinated with an immunizing dose containing 1 x 10(2) TCID50. Randomly selected vaccinated sheep mounted good cell-mediated immunity and humoral responses as measured by glucose utilization test and serum neutralization test, respectively, for the study period of 6 months.
ISSN:0049-4747
1573-7438
DOI:10.1023/B:TROP.0000026661.88631.50