Effect of an epoxy derivative of 2′5′-trioligoadenylate on human neuroblastoma cells

We studied the effect of an epoxy derivative of dephosphorylated 2[variant prime],5[variant prime]-trioligoadenylate (5[variant prime],5[variant prime]ApApAepoxy) resistive to the action of cellular phosphodiesterase on cells of human neuroblastoma IMR 32 cultured in vitro . Twenty-two hours after the addition of 5[dot]10 -6 M 2[variant prime],5[variant prime]ApApAepoxy to the culture medium, the number of cells decreased by 20% ( P < 0.05), while the content of protein in these cells increased, on average, by 52% ( P < 0.01), as compared with the control. The activities of Na + ,K + -and Ca 2+ , Mg 2+ -ATPases in a microsomal fraction obtained from cells cultured in the presence of 2[variant prime], 5[variant prime] ApApAepoxy decreased by 50% ( P < 0.001) as compared with those in the control cells. Our data indicate that 2[variant prime],5[variant prime]ApApAepoxy possess antiproliferative activity. According to our findings, the antiproliferative effect of 2[variant prime],5[variant prime] ApApAepoxy can, to a great extent, be explained by the fact that this oligoadenylate derivative significantly modulates the activities of Na + ,K + -and Ca 2+ ,Mg 2+ -ATPases. [PUBLICATION ABSTRACT]