Palladium nanocluster-based fluorescent sensing platform via synergistic effects of inner filter effect and agglomeration-induced quenching for myoglobin determination

The monomeric hemeprotein myoglobin (Mb) has immense biological importance as it is first released from damaged muscles in case of an injury. Since excessive release of Mb damages kidney and causes acute renal failure, monitoring of Mb is relevant. A novel and cost-effective fluorescence sensor for label free determination of Mb has been developed. The turn-off assay is based on methionine stabilized palladium nanoclusters (Met-PdNCs). The Met-PdNCs have been characterized by spectroscopic techniques such as fluorescence, UV–Vis spectroscopy, FTIR and microscopic technique TEM along with DLS. The quenching mechanism was attributed to the synergistic effects of agglomeration-induced quenching and inner filter effect. The developed sensor exhibited a linear range of 1.00 × 10 −5 M to 8.00 × 10 −7 M with a limit of detection of 1.67 × 10 −7 M. The practicality of the developed Mb sensor was validated through spike recovery analysis and a classic spectrophotometric method. Graphical abstract