StemForte Plus® for stem cell activation and telomerase activity for anti-aging purposes

[...]there is a need for a safer and effective treatment option for stem cell modulators for tissue regeneration and growth. Material and methods Test system details (bone marrow cells and cell line) For cytotoxicity test, Caco2 cell line (ATCC®HTB-37™, human colon epithelial cells) purchased from National Centre for Cell Sciences was used. The rinsing solution was removed by inversion of the plate and blot dry on paper towels. 250 μL of 25 μg/mL neutral red (NR) dye was added in complete medium to all wells (including the blanks) and incubated at (37°C ±1°C, ≥90% humidity, 5.0% ± 0.5 % CO2/air) for 3.0 hours ± 0.1 h (37°C ±1°C, ≥90% humidity, 5.0% ± 0.5 % CO2/air). After incubation, the NR medium was removed and cells were rinsed carefully with 250 μL/well pre-warmed D-PBS. 100 μL NR desorb solution (freshly prepared 49 parts water + 50 parts ethanol + 1-part glacial acetic acid) was added to all wells (including blanks) to extract the dye [15,16,17].