Gene expression responses in zebrafish to short-term high-hydrostatic pressure

For every 10 m of descent in the ocean, the hydrostatic pressure increases, on average, by about one atmosphere (atm, ~0.1 MPa). [...]deep seas are a formidable challenge for human exploration as well as marine fish distribution (Young et al., 1997). Zebrafish are a model species distributed in shallow waters (Beliaeva et al., 2010; Spence et al., 2006). [...]we chose the AB zebrafish line to test responses to high hydrostatic pressure. The significantly enriched GO terms were related to transmembrane transport (e.g., "regulation of transport" and "metal ion transmembrane transporter activity"), activator activity (e.g., "GABA receptor activity" and "enzyme activator activity"), molecular binding (e.g., "GTPase binding" and "calmodulin binding"), synaptic signaling (e.g., "anterograde trans-synaptic signaling" and "gamma-aminobutyric acid signaling pathway"), and protein complex oligomerization (Supplementary Table S8). [...]817 up-regulated and 1 720 down-regulated DEGs were induced in the 9 MPa-treated group compared with the 0.1 MPa-treated control. Results showed that the Spearman rank correlation coefficient in the control group was significantly higher than that in the hydrostatic pressure treatment group (Figure 1F), suggesting that the samples showed higher differential expression as pressure increased.