2-Phenyl substituted Benzimidazole derivatives: Design, synthesis, and evaluation of their antiproliferative and antimicrobial activities

The inability to meet the desired outcomes of anticancer treatment and decrease in treatment success of bacterial and fungal infections accelerated research in these areas. Our research group has conducted numerous studies, especially on benzimidazole ring systems’ antiproliferative and antimicrobial activities. In this study, the antiproliferative activity of benzimidazole compounds was tested against A549, A498, HeLa, A375, and HepG2 cancer cell lines by MTT assay. All compounds exhibited good to potent antiproliferative activity against all tested cancer cell lines. Compounds 6-chloro-2-(4-fluorobenzyl)-1 H -benzo[d]imidazole (30) and 6-chloro-2-phenethyl-1 H -benzo[d]imidazole (46) were especially active against HeLa and A375 cancer cell lines with IC 50 values in the range of 0.02–0.04 µM. In contrast, compounds 6-chloro-2-((p-tolyloxy)methyl)-1 H -benzo[d]imidazole (67) and 5(6)-chloro-2-((4-hydroxyphenoxy)methyl)-1 H -benzimidazole (68) were active against A549 and A498 cancer cell lines with an IC 50 value of 0.08 µM. These compounds ( 30 , 46 , 67 , and 68 ) were less toxic to normal human cells than the positive control compound methotrexate, which was screened to determine its toxicity against normal cell lines (HEK293). In the second part of the study, all compounds were tested to demonstrate their antimicrobial properties. All compounds exhibited moderate activity against all tested bacteria and fungi. However, some phenoxy methyl derivatives 5-chloro-2-((4-chlorophenoxy)methyl)-1 H -benzo[d]imidazole ( 69 ) and 5,6-dichloro-2-((4-chlorophenoxy)methyl)-1 H -benzo[d]imidazole and ( 74 ) were most active against Candida (