Mercury-Induced Inhibition of Tyrosine Phosphorylation of Sperm Proteins and Altered Functional Dynamics of Buck Spermatozoa: an In Vitro Study
Present study was undertaken on buck spermatozoa to investigate the effect of mercuric chloride on functional dynamics of buck spermatozoa. Four different concentrations (0.031, 0.125, 0.25 and 1.25 μg/mL) of mercuric chloride, which were 1/40 th , 1/10 th , 1/5 th and equivalent to the LC 50 value...
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| Veröffentlicht in: | Biological trace element research 2020-12, Vol.198 (2), p.478-492 |
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| creator | Kushawaha, Bhawna Yadav, Rajkumar Singh Swain, Dilip Kumar Rai, Pradeep K Garg, Satish Kumar |
| description | Present study was undertaken on buck spermatozoa to investigate the effect of mercuric chloride on functional dynamics of buck spermatozoa. Four different concentrations (0.031, 0.125, 0.25 and 1.25 μg/mL) of mercuric chloride, which were 1/40
th
, 1/10
th
, 1/5
th
and equivalent to the LC
50
value of HgCl
2
, were selected for studying their effect following in vitro exposure for 15 min and 3 h. Exposure of spermatozoa to 0.031 μg/mL mercuric chloride for 3 h resulted in significant (
p
|
| doi_str_mv | 10.1007/s12011-020-02077-z |
| format | Article |
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th
, 1/10
th
, 1/5
th
and equivalent to the LC
50
value of HgCl
2
, were selected for studying their effect following in vitro exposure for 15 min and 3 h. Exposure of spermatozoa to 0.031 μg/mL mercuric chloride for 3 h resulted in significant (
p
< 0.05) decrease in sperm motility, sperm having intact membrane, intact acrosome and high mitochondrial trans-membrane potential. However, following exposure to higher concentrations (0.25, 1.25 μg/mL), similar results were observed even after 15 min of exposure. HgCl
2
significantly (
p
< 0.05) increased the levels of malondialdehyde and reactive oxygen species and significantly (
p
< 0.05) decreased total antioxidant capacity and superoxide dismutase activity in spermatozoa within 15 min of exposure. Mercuric chloride-treated spermatozoa did not show capacitation, rather exhibited spontaneous acrosome reaction along with significant increase in intracellular Ca
2+
and cAMP levels. Immuno-blotting of semen samples of control and 0.031 μg/mL mercury-treated groups showed low intensity bands of p55, p70, p80, p105 and p190 kDa tyrosine phosphorylation proteins while higher concentration-treated groups showed no such bands. Our findings evidently suggest that mercuric chloride even at 0.031 μg/mL adversely affected sperm functions, inhibited tyrosine phosphorylation proteins and capacitation due to oxidative stress. Spontaneous acrosome reaction (AR) in mercury-treated spermatozoa may possibly be due to increase in intracellular Ca
2+
and cAMP levels, and capacitation failure may be due to inhibition of tyrosine phosphorylation of proteins.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-020-02077-z</identifier><identifier>PMID: 32064576</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acrosome reaction ; Antioxidants ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Calcium (intracellular) ; Calcium ions ; Capacitation ; Chloride ; Chlorides ; Cyclic AMP ; Dynamics ; Exposure ; Intracellular ; Life Sciences ; Membrane potential ; Membranes ; Mercuric chloride ; Mercury ; Mercury (metal) ; Mercury compounds ; Mitochondria ; Mortality causes ; Nutrition ; Oncology ; Oxidative stress ; Phosphorylation ; Proteins ; Reactive oxygen species ; Semen ; Sperm ; Spermatozoa ; Superoxide dismutase ; Toxicity tests ; Tyrosine</subject><ispartof>Biological trace element research, 2020-12, Vol.198 (2), p.478-492</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a9cc8106c2ec22be753d3895c8bba719cdf0fce114bbfd90895ad70b78713e763</citedby><cites>FETCH-LOGICAL-c375t-a9cc8106c2ec22be753d3895c8bba719cdf0fce114bbfd90895ad70b78713e763</cites><orcidid>0000-0002-6178-7068</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-020-02077-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-020-02077-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32064576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kushawaha, Bhawna</creatorcontrib><creatorcontrib>Yadav, Rajkumar Singh</creatorcontrib><creatorcontrib>Swain, Dilip Kumar</creatorcontrib><creatorcontrib>Rai, Pradeep K</creatorcontrib><creatorcontrib>Garg, Satish Kumar</creatorcontrib><title>Mercury-Induced Inhibition of Tyrosine Phosphorylation of Sperm Proteins and Altered Functional Dynamics of Buck Spermatozoa: an In Vitro Study</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>Present study was undertaken on buck spermatozoa to investigate the effect of mercuric chloride on functional dynamics of buck spermatozoa. Four different concentrations (0.031, 0.125, 0.25 and 1.25 μg/mL) of mercuric chloride, which were 1/40
th
, 1/10
th
, 1/5
th
and equivalent to the LC
50
value of HgCl
2
, were selected for studying their effect following in vitro exposure for 15 min and 3 h. Exposure of spermatozoa to 0.031 μg/mL mercuric chloride for 3 h resulted in significant (
p
< 0.05) decrease in sperm motility, sperm having intact membrane, intact acrosome and high mitochondrial trans-membrane potential. However, following exposure to higher concentrations (0.25, 1.25 μg/mL), similar results were observed even after 15 min of exposure. HgCl
2
significantly (
p
< 0.05) increased the levels of malondialdehyde and reactive oxygen species and significantly (
p
< 0.05) decreased total antioxidant capacity and superoxide dismutase activity in spermatozoa within 15 min of exposure. Mercuric chloride-treated spermatozoa did not show capacitation, rather exhibited spontaneous acrosome reaction along with significant increase in intracellular Ca
2+
and cAMP levels. Immuno-blotting of semen samples of control and 0.031 μg/mL mercury-treated groups showed low intensity bands of p55, p70, p80, p105 and p190 kDa tyrosine phosphorylation proteins while higher concentration-treated groups showed no such bands. Our findings evidently suggest that mercuric chloride even at 0.031 μg/mL adversely affected sperm functions, inhibited tyrosine phosphorylation proteins and capacitation due to oxidative stress. Spontaneous acrosome reaction (AR) in mercury-treated spermatozoa may possibly be due to increase in intracellular Ca
2+
and cAMP levels, and capacitation failure may be due to inhibition of tyrosine phosphorylation of proteins.</description><subject>Acrosome reaction</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Calcium (intracellular)</subject><subject>Calcium ions</subject><subject>Capacitation</subject><subject>Chloride</subject><subject>Chlorides</subject><subject>Cyclic AMP</subject><subject>Dynamics</subject><subject>Exposure</subject><subject>Intracellular</subject><subject>Life Sciences</subject><subject>Membrane potential</subject><subject>Membranes</subject><subject>Mercuric chloride</subject><subject>Mercury</subject><subject>Mercury (metal)</subject><subject>Mercury compounds</subject><subject>Mitochondria</subject><subject>Mortality causes</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Oxidative stress</subject><subject>Phosphorylation</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Semen</subject><subject>Sperm</subject><subject>Spermatozoa</subject><subject>Superoxide dismutase</subject><subject>Toxicity 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K</au><au>Garg, Satish Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mercury-Induced Inhibition of Tyrosine Phosphorylation of Sperm Proteins and Altered Functional Dynamics of Buck Spermatozoa: an In Vitro Study</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><addtitle>Biol Trace Elem Res</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>198</volume><issue>2</issue><spage>478</spage><epage>492</epage><pages>478-492</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>Present study was undertaken on buck spermatozoa to investigate the effect of mercuric chloride on functional dynamics of buck spermatozoa. Four different concentrations (0.031, 0.125, 0.25 and 1.25 μg/mL) of mercuric chloride, which were 1/40
th
, 1/10
th
, 1/5
th
and equivalent to the LC
50
value of HgCl
2
, were selected for studying their effect following in vitro exposure for 15 min and 3 h. Exposure of spermatozoa to 0.031 μg/mL mercuric chloride for 3 h resulted in significant (
p
< 0.05) decrease in sperm motility, sperm having intact membrane, intact acrosome and high mitochondrial trans-membrane potential. However, following exposure to higher concentrations (0.25, 1.25 μg/mL), similar results were observed even after 15 min of exposure. HgCl
2
significantly (
p
< 0.05) increased the levels of malondialdehyde and reactive oxygen species and significantly (
p
< 0.05) decreased total antioxidant capacity and superoxide dismutase activity in spermatozoa within 15 min of exposure. Mercuric chloride-treated spermatozoa did not show capacitation, rather exhibited spontaneous acrosome reaction along with significant increase in intracellular Ca
2+
and cAMP levels. Immuno-blotting of semen samples of control and 0.031 μg/mL mercury-treated groups showed low intensity bands of p55, p70, p80, p105 and p190 kDa tyrosine phosphorylation proteins while higher concentration-treated groups showed no such bands. Our findings evidently suggest that mercuric chloride even at 0.031 μg/mL adversely affected sperm functions, inhibited tyrosine phosphorylation proteins and capacitation due to oxidative stress. Spontaneous acrosome reaction (AR) in mercury-treated spermatozoa may possibly be due to increase in intracellular Ca
2+
and cAMP levels, and capacitation failure may be due to inhibition of tyrosine phosphorylation of proteins.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32064576</pmid><doi>10.1007/s12011-020-02077-z</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-6178-7068</orcidid></addata></record> |
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| identifier | ISSN: 0163-4984 |
| ispartof | Biological trace element research, 2020-12, Vol.198 (2), p.478-492 |
| issn | 0163-4984 1559-0720 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Acrosome reaction Antioxidants Biochemistry Biomedical and Life Sciences Biotechnology Calcium (intracellular) Calcium ions Capacitation Chloride Chlorides Cyclic AMP Dynamics Exposure Intracellular Life Sciences Membrane potential Membranes Mercuric chloride Mercury Mercury (metal) Mercury compounds Mitochondria Mortality causes Nutrition Oncology Oxidative stress Phosphorylation Proteins Reactive oxygen species Semen Sperm Spermatozoa Superoxide dismutase Toxicity tests Tyrosine |
| title | Mercury-Induced Inhibition of Tyrosine Phosphorylation of Sperm Proteins and Altered Functional Dynamics of Buck Spermatozoa: an In Vitro Study |
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