Response surface methodology (RSM) for assessing the effects of pretreatment, feedstock, and enzyme complex association on cellulose hydrolysis

The enzymatic hydrolysis of sugarcane bagasse cellulose (SBC) was performed after acid/peroxide-alkali (APA) pretreatment, soda/AQ pulping, and bleaching steps for producing glucose. Cellic® CTec3 (CT) and Celluclast®1.5L (CL) complexes were evaluated in both individual and mixed systems for observi...

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Veröffentlicht in:Biomass conversion and biorefinery 2022-07, Vol.12 (7), p.2811-2822
Hauptverfasser: Campos, Leila M. A., Moura, Heloise O. M. A., Cruz, Antonio J. G., Assumpção, Samira M. N., de Carvalho, Luciene S., Pontes, Luiz A. M.
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Sprache:eng
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Zusammenfassung:The enzymatic hydrolysis of sugarcane bagasse cellulose (SBC) was performed after acid/peroxide-alkali (APA) pretreatment, soda/AQ pulping, and bleaching steps for producing glucose. Cellic® CTec3 (CT) and Celluclast®1.5L (CL) complexes were evaluated in both individual and mixed systems for observing their influence in cellulose conversion. Deconvolution of XRD patterns and SEM images proved the low crystallinity and high accessibility of SBC, thus being able to provide significantly higher glucose yield than the commercial cellulose with predominant Iβ polymorphism. The analysis of variance (ANOVA) and the response surface methodology (RSM), applied to a full factorial 2 4 design of experiments built for assessing the activity of CT and CL enzymes together in the hydrolytic system, were accurate in describing that the feedstock (SBC or commercial cellulose) and the treatment of cellulose are the factors with major effect on the saccharification efficiency ( F  = 744.12, p  
ISSN:2190-6815
2190-6823
DOI:10.1007/s13399-020-00756-4