Horseradish Peroxidase: Analytical Capabilities in the Determination of Zearalenone

Horseradish Peroxidase: Analytical Capabilities in the Determination of Zearalenone

The possibility of using the horseradish peroxidase (HRP)–phenol enzyme–substrate system in the development of an amperometric immunoenzyme sensor for the determination of the mycotoxin zearalenone (ZEA) was shown. New immunoenzyme amperometric sensors based on printed graphite electrodes, including...

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Veröffentlicht in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2022-06, Vol.77 (6), p.671-680
Hauptverfasser: Medyantseva, E. P., Beilinson, R. M., Nikolaenko, A. I., Budnikov, H. C.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Deoxynivalenol
Electrical measurement
Food
Nanoparticles
Peroxidase
Sensors
Substrates
Zearalenone
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Zusammenfassung:The possibility of using the horseradish peroxidase (HRP)–phenol enzyme–substrate system in the development of an amperometric immunoenzyme sensor for the determination of the mycotoxin zearalenone (ZEA) was shown. New immunoenzyme amperometric sensors based on printed graphite electrodes, including those modified with a fullerene/gold nanoparticles and HRP composite, were proposed for the determination of ZEA in a concentration range from 1 × 10 –11 to 1 × 10 –6 M, and c d was 5 × 10 –12 M The binding constant of immune complexes ( K a = (5.3 ± 0.2) × 10 8 mol –1 ) and the percentage of cross reactions, which was
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934822060090