Biliary polycyclic aromatic hydrocarbons and enzymatic biomarkers in Eugerres brasilianus along four tropical estuaries

Polycyclic aromatic hydrocarbons (PAH) in bile and biochemical biomarkers were evaluated in Brazilian mojarra Eugerres brasilianus along four estuaries in northeastern Brazil. Bile PAHs naphthalene, phenanthrene chrysene, pyrene and benzo[a]pyrene were lowest at Formoso River Estuarine System (FRES), an area with low population density focused on tourism. Fish sampled in Suape Estuarine Complex (SEC), where a growing industrial port complex is established indicated higher naphthalene and pyrene concentrations compared with FRES. Fish sampled in highly urbanized and populated Bacia do Pina Estuarine Complex (BPEC) and Barra de Jangada Estuarine System (BJES) indicated an increase in all PAHs compared to FRES. Activities of phase 1 Ethoxyresorufin-O-deethylase, phase 2 glutathione-S-transferase and antioxidant defense catalase were induced up to 20, 2 and 2-fold in BJES and BPEC compared to FRES. This study confirms E. brasilianus as an important sentinel species, providing baseline information for these tropical estuaries with different degrees of anthropogenic pressure. •Fish bile PAHs and biochemical biomarkers ranked impact in four tropical estuaries.•Induction of biomarkers EROD, GST and CAT correlated with increasing bile PAHs.•Biomarkers in Eugerres brasilianus from Ariquindá River can serve as a local baseline.•Eugerres brasilianus is a useful sentinel species to monitor tropical estuaries.