DNA Origami‐Based Protein Manipulation Systems: From Function Regulation to Biological Application

Proteins directly participate in tremendous physiological processes and mediate a variety of cellular functions. However, precise manipulation of proteins with predefined relative position and stoichiometry for understanding protein‐protein interactions and guiding cellular behaviors is still challenging. With superior programmability of DNA molecules, DNA origami technology is able to construct arbitrary nanostructures that can accurately control the arrangement of proteins with various functionalities to solve these problems. Herein, starting from the classification of DNA origami nanostructures and the category of assembled proteins, we summarize the existing DNA origami‐based protein manipulation systems (PMSs), review the advances on the regulation of their functions, and discuss their applications in cellular behavior modulation and disease therapy. Moreover, the limitations and potential directions of DNA origami‐based PMSs are also presented, which may offer guidance for rational construction and ingenious application. DNA origami creates an ideal operation panel for positioning and manipulating individual proteins with nanoscale precision. This review provides an overview of the existing DNA origami‐based protein manipulation systems for protein function regulation and biological applications.