A biphasic material combined with injectable platelet-rich fibrin for the potential regeneration of oral soft and hard tissues

The irreversible bone resorption accompanied by atrophy and reconstruction of soft tissue around the tooth loss site is still a challenging problem for implant therapy. The present study describes biphasic materials composed of fish-collagen/poly (lactide-co-glycolide) electrospun membranes and nano-hydroxyapatite/poly (lactide-co-glycolide) scaffolds for the regeneration of oral hard and soft tissues. Meanwhile, the injectable platelet-rich fibrin (i-PRF) from autologous blood was prepared and characterized for combined application. The in-vitro experimental results confirmed that the fabricated biphasic material had high cytocompatibility and could enhance the in-vitro osteogenic differentiation. Moreover, an animal model of oral simultaneous tissue defects in the palate of rabbits was established to evaluate the repair efficiency of the biphasic materials combined with i-PRF. It was found to have good biocompatibility and regeneration activity in vivo after the implantation into the defects areas in rabbits, and the combination of i-PRF with biphasic materials was more favored for oral simultaneous tissue regeneration. Conclusively, this research suggests that the fabricated biphasic materials combined with i-PRF may have a promising prospect for the simultaneous regeneration of oral soft and hard tissues. Graphical abstract