A Microtiter-Plate Chemiluminescence Method for the Determination of MicroRNA-141 Based on the Application of Catalytic Hairpin Assembly and a Streptavidin–Polyperoxidase Conjugate

Currently, microRNAs attract increased attention of researchers as promising markers of various human diseases. This work presents a heterogeneous chemiluminescence method for the quantitative determination of cancer marker microRNA-141, based on an enzyme-free amplification reaction, given the name catalytic hairpin assembly (CHA). To additionally enhance the sensitivity of the method, a detection system with the double amplification of the signal, including the use of a streptavidin–horseradish polyperoxidase conjugate and an enhanced chemiluminescence reaction, was used. Because of the use of the triple amplification strategy, the method is characterized by the high sensitivity and the low limit of detection. It was found that this method for the determination of microRNA-141 is characterized by high specificity through the use of CHA. The specified features of the method suggest its successful application to clinical diagnostics.