Effects of transcranial magnetoacoustic electrical stimulation on calcium signaling in prefrontal cortex neural clusters

Transcranial magnetoacoustic and electrical stimulation (TMAES), as a new type of brain nerve regulation and research method, uses the induced currents generated by the coupling of ultrasound and magnetic fields to regulate the neural electrical activity in different brain regions. As the second envoy of nerve signal, calcium ion plays a key role in nerve signal transmission. In order to explore the effect of transcranial magnetoacoustic electrical stimulation on the discharge activity of the prefrontal cortex, 15 mice were divided into the control group, the ultrasound group and the magnetoacoustic group. 0.3 T stimulation, the ultrasound group received only the same intensity of ultrasound stimulation, and the control group received no ultrasound and magnetic field for one week. The concentration of calcium ions in the prefrontal cortex during stimulation was recorded in real time by optical fiber photometry, and a novel object recognition experiment was performed to compare the behavioral differences and t