Simultaneous Enantioseparation and Rapid Determination of Atenolol and Amlodipine Besylate by Capillary Electrochromatography

Simultaneous enantioseparation and rapid determination method of atenolol (AN) and amlodipine besylate (AMB) was firstly established using diphenylmethane diisocyanate-β-CD-modified COF (MDI-β-CD-modified COF) as chiral solid phases (CSP) by capillary electrochromatography (CEC). The optimal separation conditions were explored and the concentration of the acetate buffer solution was 20 mmol/L, pH = 5.0, operating voltage 20 kV, column temperature 20 °C, and then the four enantiomers of AN and AMB were baseline separated less than 5 min. The method validation was assessed by applying standard addition technique. The results showed that the concentration of each enantiomer exhibited a good linear relationship with the chromatographic peak area ( r 2 > 0.9918); the limit of detection (LOD) was 0.55–0.98 mg/L; the limit of quantification (LQD) was 1.83–3.27 mg/L; the average recoveries were in the range of 95.4–103.0%; the relative standard deviation (RSD) was less than 3.8%. The method established is rapid, simple, sensitive, accurate and reliable, and it can be used for routine analysis and quality control of the two chiral drugs separately or in combinations.