ROLE OF HSA-MIR-451A ON PROGRESSION OF HUMAN BREAST CANCER

Breast cancer is a global concern related to public health with an average of 2.200.000 global new cases and close to 7% of world deaths related to cancer. Breast cancer, as other solid tumours, behave generally in steps described as proliferation, migration, and invasion which are regulated by tumour microenvironment, a specialized network promoting cancer cells which finally will achieve stabilization with support of signalling pathways. Within the great variety of molecules and the reported dysregulation of microRNAs (miRNAs) in tumour progression, has attracted attention, and based on previous reports, how highly expressed placental miRNAs are related in specific signalling pathways which in placenta are under control but they are not in cancer. The aim of this research was to evaluate the role of hsa-mir-451a in breast cancer progression. Human MCF-7 cell line was cultured in Dulbecco`s modified Eagle`s medium and transfected with hsamir- 451a mimic. Cell line transfected was evaluated for classical tumour progression assays as, colony formation, proliferation, invasion, and migration. The results showed that cell lines transfected decreased their abilities to progress in vitro, revealing reduced capacity to form colonies (p