An in-vitro antibacterial activity of Aloe vera and gentamicin against Escherichia coli and Klebsiella pneumoniae isolates from mastitis milk samples, from Tandojam, Sindh Pakistan

The current study was conducted to examine the in vitro antibacterial activity of pure Aloe vera extract and compared with commonly used antibiotic, Gentamicin against Escherichia coli and Klebsiella pneumonia isolated from mastitis milk. For this purpose, 50 milk samples were collected from dairy farms in the vicinity of Tandojam and processed the samples for culture, identification, and Minimum inhibitory concentration of pure Aloe vera extract and gentamicin in the laboratory of department of Veterinary Pharmacology, Sindh Agriculture University Tandojam, to record the MIC against isolated organisms at different concentrations i-e 40, 20, 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.081 and 0.039µl. The MIC of the isolates was determined by the turbidity and translucency of the cultured medium Results of present study revealed that out of 50 milk samples, 35 (70%) and 15 (30%) were found positive for E. coli and K. pneumonia, respectively. It was found that E. coli stopped growth at 10µl of pure Aloe vera and 1.25µl of Gentamicin. Whereas K. pneumoniae failed to grow at 2.5µl of pure Aloe vera, but it was found highly susceptible for gentamicin and stopped growth at lowest conc: i-e 0.03µl. The comparative results revealed that the concentrations of Aloe vera used in this study were not as effective as that of commercial product, but Aloe vera stopped the growth of isolated organisms at certain level as that of gentamicin. Therefore, due to emergence of resistance towards antibiotics, Aloe vera can be used as a substitute of antibiotics.