Cloning and Expression Analysis of Sesquiterpene Synthase Gene SgSTPS3 in Sindora glabra
[Objective] The oleoresin in the stem of Sindora glabra contains rich sesquiterpenoids, while terpene synthase is one of the key enzymes in the terpene biosynthesis pathway. Cloning and expression analysis of S.glabra sesquiterpene synthase gene SgSTPS3 is helpful to explore the formation mechanism...
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| Veröffentlicht in: | Linye kexue (1979) 2021-01, Vol.57 (12), p.68 |
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| container_issue | 12 |
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| container_title | Linye kexue (1979) |
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| creator | Chen, Zhaoli Yu, Niu Li, Rongsheng Zou, Wentao Dong, Mingliang Zhu, Maocheng Yang, Jinchang |
| description | [Objective] The oleoresin in the stem of Sindora glabra contains rich sesquiterpenoids, while terpene synthase is one of the key enzymes in the terpene biosynthesis pathway. Cloning and expression analysis of S.glabra sesquiterpene synthase gene SgSTPS3 is helpful to explore the formation mechanism of S.glabra oleoresin, which will provide a new theoretical basis for analyzing the mechanisms of terpenoid biosynthesis and metabolism in forest trees. [Method] Based on the previous transcriptome data, the sesquiterpene synthase gene SgSTPS3 was cloned from the stem of mature S.glabra, and analyzed by bioinformatics tools. Prokaryotic expression in Escherichia coli cells, and identification by-products by GC-MS. The expression levels of this gene at different ages(0.5, 1.5, 15, 30 years old) and in differet organs(root, phloem, xylem, tender leaves, old leaves) of S.glabra were also analyzed by quantitative real-time PCR. To analyze the response pattern of this gene to hormones, salicylic acid(SA), methyl jasmona |
| format | Article |
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Cloning and expression analysis of S.glabra sesquiterpene synthase gene SgSTPS3 is helpful to explore the formation mechanism of S.glabra oleoresin, which will provide a new theoretical basis for analyzing the mechanisms of terpenoid biosynthesis and metabolism in forest trees. [Method] Based on the previous transcriptome data, the sesquiterpene synthase gene SgSTPS3 was cloned from the stem of mature S.glabra, and analyzed by bioinformatics tools. Prokaryotic expression in Escherichia coli cells, and identification by-products by GC-MS. The expression levels of this gene at different ages(0.5, 1.5, 15, 30 years old) and in differet organs(root, phloem, xylem, tender leaves, old leaves) of S.glabra were also analyzed by quantitative real-time PCR. To analyze the response pattern of this gene to hormones, salicylic acid(SA), methyl jasmona</description><identifier>ISSN: 1001-7488</identifier><language>chi</language><publisher>Beijing: Chinese Society of Forestry</publisher><subject>Age ; Amino acids ; Biochemical analysis ; Bioinformatics ; Biosynthesis ; Cloning ; Copaifera ; E coli ; Enzymes ; Gene expression ; Homology ; Hormones ; Leaves ; Linalool ; Metabolism ; Methyl jasmonate ; Oleoresins ; Organs ; Pattern analysis ; Phloem ; Salicylic acid ; Seedlings ; Sesquiterpene synthase ; Sesquiterpenoids ; Sindora glabra ; Spraying ; Stems ; Substrates ; Terpene synthase ; Transcriptomes ; Xylem</subject><ispartof>Linye kexue (1979), 2021-01, Vol.57 (12), p.68</ispartof><rights>Copyright Chinese Society of Forestry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Chen, Zhaoli</creatorcontrib><creatorcontrib>Yu, Niu</creatorcontrib><creatorcontrib>Li, Rongsheng</creatorcontrib><creatorcontrib>Zou, Wentao</creatorcontrib><creatorcontrib>Dong, Mingliang</creatorcontrib><creatorcontrib>Zhu, Maocheng</creatorcontrib><creatorcontrib>Yang, Jinchang</creatorcontrib><title>Cloning and Expression Analysis of Sesquiterpene Synthase Gene SgSTPS3 in Sindora glabra</title><title>Linye kexue (1979)</title><description>[Objective] The oleoresin in the stem of Sindora glabra contains rich sesquiterpenoids, while terpene synthase is one of the key enzymes in the terpene biosynthesis pathway. Cloning and expression analysis of S.glabra sesquiterpene synthase gene SgSTPS3 is helpful to explore the formation mechanism of S.glabra oleoresin, which will provide a new theoretical basis for analyzing the mechanisms of terpenoid biosynthesis and metabolism in forest trees. [Method] Based on the previous transcriptome data, the sesquiterpene synthase gene SgSTPS3 was cloned from the stem of mature S.glabra, and analyzed by bioinformatics tools. Prokaryotic expression in Escherichia coli cells, and identification by-products by GC-MS. The expression levels of this gene at different ages(0.5, 1.5, 15, 30 years old) and in differet organs(root, phloem, xylem, tender leaves, old leaves) of S.glabra were also analyzed by quantitative real-time PCR. To analyze the response pattern of this gene to hormones, salicylic acid(SA), methyl jasmona</description><subject>Age</subject><subject>Amino acids</subject><subject>Biochemical analysis</subject><subject>Bioinformatics</subject><subject>Biosynthesis</subject><subject>Cloning</subject><subject>Copaifera</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Homology</subject><subject>Hormones</subject><subject>Leaves</subject><subject>Linalool</subject><subject>Metabolism</subject><subject>Methyl jasmonate</subject><subject>Oleoresins</subject><subject>Organs</subject><subject>Pattern analysis</subject><subject>Phloem</subject><subject>Salicylic acid</subject><subject>Seedlings</subject><subject>Sesquiterpene synthase</subject><subject>Sesquiterpenoids</subject><subject>Sindora glabra</subject><subject>Spraying</subject><subject>Stems</subject><subject>Substrates</subject><subject>Terpene synthase</subject><subject>Transcriptomes</subject><subject>Xylem</subject><issn>1001-7488</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqNjcsKgkAUQGdRkJT_cKG1MObgYxlitQzGRTsZcbQJuaNzFfLvk-gDWh0OHDgb5oWch0Ei0nTHfCJT84iHmUh55rFH3ls02IHCBor34PQaWIQzqn4hQ2BbkJrG2UzaDRo1yAWnpyIN1691srzLCAyCNNhYp6DrVe3UgW1b1ZP2f9yz46Uo81swODvOmqbqZWe3Xqg6xULwJImSOPqv-gCQ1kGc</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Chen, Zhaoli</creator><creator>Yu, Niu</creator><creator>Li, Rongsheng</creator><creator>Zou, Wentao</creator><creator>Dong, Mingliang</creator><creator>Zhu, Maocheng</creator><creator>Yang, Jinchang</creator><general>Chinese Society of Forestry</general><scope>7SN</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20210101</creationdate><title>Cloning and Expression Analysis of Sesquiterpene Synthase Gene SgSTPS3 in Sindora glabra</title><author>Chen, Zhaoli ; Yu, Niu ; Li, Rongsheng ; Zou, Wentao ; Dong, Mingliang ; Zhu, Maocheng ; Yang, Jinchang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_26440773763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2021</creationdate><topic>Age</topic><topic>Amino acids</topic><topic>Biochemical analysis</topic><topic>Bioinformatics</topic><topic>Biosynthesis</topic><topic>Cloning</topic><topic>Copaifera</topic><topic>E coli</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Homology</topic><topic>Hormones</topic><topic>Leaves</topic><topic>Linalool</topic><topic>Metabolism</topic><topic>Methyl jasmonate</topic><topic>Oleoresins</topic><topic>Organs</topic><topic>Pattern analysis</topic><topic>Phloem</topic><topic>Salicylic acid</topic><topic>Seedlings</topic><topic>Sesquiterpene synthase</topic><topic>Sesquiterpenoids</topic><topic>Sindora glabra</topic><topic>Spraying</topic><topic>Stems</topic><topic>Substrates</topic><topic>Terpene synthase</topic><topic>Transcriptomes</topic><topic>Xylem</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Zhaoli</creatorcontrib><creatorcontrib>Yu, Niu</creatorcontrib><creatorcontrib>Li, Rongsheng</creatorcontrib><creatorcontrib>Zou, Wentao</creatorcontrib><creatorcontrib>Dong, Mingliang</creatorcontrib><creatorcontrib>Zhu, Maocheng</creatorcontrib><creatorcontrib>Yang, Jinchang</creatorcontrib><collection>Ecology Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Linye kexue (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Zhaoli</au><au>Yu, Niu</au><au>Li, Rongsheng</au><au>Zou, Wentao</au><au>Dong, Mingliang</au><au>Zhu, Maocheng</au><au>Yang, Jinchang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Expression Analysis of Sesquiterpene Synthase Gene SgSTPS3 in Sindora glabra</atitle><jtitle>Linye kexue (1979)</jtitle><date>2021-01-01</date><risdate>2021</risdate><volume>57</volume><issue>12</issue><spage>68</spage><pages>68-</pages><issn>1001-7488</issn><abstract>[Objective] The oleoresin in the stem of Sindora glabra contains rich sesquiterpenoids, while terpene synthase is one of the key enzymes in the terpene biosynthesis pathway. Cloning and expression analysis of S.glabra sesquiterpene synthase gene SgSTPS3 is helpful to explore the formation mechanism of S.glabra oleoresin, which will provide a new theoretical basis for analyzing the mechanisms of terpenoid biosynthesis and metabolism in forest trees. [Method] Based on the previous transcriptome data, the sesquiterpene synthase gene SgSTPS3 was cloned from the stem of mature S.glabra, and analyzed by bioinformatics tools. Prokaryotic expression in Escherichia coli cells, and identification by-products by GC-MS. The expression levels of this gene at different ages(0.5, 1.5, 15, 30 years old) and in differet organs(root, phloem, xylem, tender leaves, old leaves) of S.glabra were also analyzed by quantitative real-time PCR. To analyze the response pattern of this gene to hormones, salicylic acid(SA), methyl jasmona</abstract><cop>Beijing</cop><pub>Chinese Society of Forestry</pub></addata></record> |
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| language | chi |
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| source | Alma/SFX Local Collection |
| subjects | Age Amino acids Biochemical analysis Bioinformatics Biosynthesis Cloning Copaifera E coli Enzymes Gene expression Homology Hormones Leaves Linalool Metabolism Methyl jasmonate Oleoresins Organs Pattern analysis Phloem Salicylic acid Seedlings Sesquiterpene synthase Sesquiterpenoids Sindora glabra Spraying Stems Substrates Terpene synthase Transcriptomes Xylem |
| title | Cloning and Expression Analysis of Sesquiterpene Synthase Gene SgSTPS3 in Sindora glabra |
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