Cloning and Expression Analysis of Sesquiterpene Synthase Gene SgSTPS3 in Sindora glabra

[Objective] The oleoresin in the stem of Sindora glabra contains rich sesquiterpenoids, while terpene synthase is one of the key enzymes in the terpene biosynthesis pathway. Cloning and expression analysis of S.glabra sesquiterpene synthase gene SgSTPS3 is helpful to explore the formation mechanism of S.glabra oleoresin, which will provide a new theoretical basis for analyzing the mechanisms of terpenoid biosynthesis and metabolism in forest trees. [Method] Based on the previous transcriptome data, the sesquiterpene synthase gene SgSTPS3 was cloned from the stem of mature S.glabra, and analyzed by bioinformatics tools. Prokaryotic expression in Escherichia coli cells, and identification by-products by GC-MS. The expression levels of this gene at different ages(0.5, 1.5, 15, 30 years old) and in differet organs(root, phloem, xylem, tender leaves, old leaves) of S.glabra were also analyzed by quantitative real-time PCR. To analyze the response pattern of this gene to hormones, salicylic acid(SA), methyl jasmona