Real-time impedimetric detection of cardiac troponin I using ITO-coated vertically aligned silicon nanowires

[Display omitted] •Various concentrations of cTnI in sera were impedimetrically detected by cTnIAb-modified-ITO-coated-VASiNWs.•It displays a linear range of detection (1.76011–17601.1 ng mL−1).•The devices achieves accurate detection of cTnI in real sera. This research focuses on developing a sensitive nanoelectronic device (NED) with impedimetric detection of human cardiac troponin I (cTnI) for the real-time diagnosis of acute myocardial infarction. The sensing area of NED was composed of indium tin oxide (ITO)-coated vertically aligned silicon nanowires (VASiNWs). ITO-coated-VASiNWs were examined by X-ray diffraction and scanning electron microscope for the identification of crystalline structure and morphological observation, respectively. After cTnI antibody (cTnIAb) modification, various concentrations of cTnI in the human blood sera were then impedimetrically detected by cTnIAb-modified-ITO-coated-VASiNWs via microfluidic systems. Our device exhibited a linear range from 1.76011 to 17601.1 ng/mL with good sensitivity for the detection of cTnI.