Magnetic graphite-ODA@CoFe2O4: attempting to produce and characterize the development of an innovative nanocomposite to investigate its antimicrobial properties

Graphite is a carbon-based substance with continuous layers of carbon. According to biomechanical characteristics and excellent biocompatibility nanocomposites, have been widely applied in biomedical implantable instruments. However, antibiotic resistant bacterial infections are a great challenge in medical applications of nanomedical devices. In the present study, cobalt ferrite nanomaterials were created through green synthesis methods using Rutin extracts as a reduction agent. Afterward, it was loaded on graphite (G) and graphite-octadecylamine (G-ODA) through a deposition–precipitation technique to produce novel and multi-purpose graphite-based nanocomposites, graphite@CoFe 2 O 4 (G@CoFe 2 O 4 ) and graphite-octadecylamine@CoFe 2 O 4 (G-ODA@CoFe 2 O 4 ), in order to advance in biomedical applications in addition to improving antibacterial properties and safety. Nanocomposites characteristics were evaluated by FESEM, XRD, FTIR, and VSM, in addition to assessing their antibacterial properties and cellular toxicity. FESEM results revealed uniform distribution of spherical CoFe 2 O 4 NPs on the graphene basal planes with the average size of 32.2 nm in G-ODA@CoFe 2 O 4 nanocomposites, as well as the excellent antimicrobial function against both gram-negative and gram-positive bacteria with insignificant cytotoxicity. The facile and rapid operation, as well as successful antibacterial efficiency of G-ODA@CoFe 2 O 4 nanocomposites, suggest that it could become a promising opportunity for advanced antimicrobial biomedical instruments in the future.