Identification of Interspecific Hybrids between Populus deltoides and P. simonii by Using Species-Specific InDel Marker

[Objective] Aiming at the problem that the seedling stage morphology of interspecific hybrid progeny of poplar is not easy to distinguish, the species-specific InDel locus development markers of Populus americana and Populus chinensis were used to rapidly identify the progeny of interspecific hybridization, which is the best choice for poplar and poplar The identification of interspecific hybrid progeny and molecular marker-assisted breeding of Populus cultivars provide important reference technology. [Method] Using Populus nigra, Populus lobulari and their hybrid progeny as experimental materials, the analysis was performed according to the resequencing results of P. nigra and P. lobulari. Species-specific InDel sites were used and primers were designed. Native polyacrylamide gel electrophoresis was used to test the versatility and species-specificity of primers. The selected species-specific primers were used to identify the authenticity of interspecific hybrid progeny. .[Result] The resequencing sequences