Determination of Vitamin D3 in the Fortified Sunflower Oil: Comparison of Two Developed Methods

Herein, the current investigation presents the study of two novel methods namely saponification liquid–liquid extraction coupled TLC (Sp-LLE-TLC) and direct liquid–liquid microextraction (LLME). These two methods were developed for enhanced determination of vitamin D 3 in the fortified sunflower oil prior HPLC–UV analysis. By employing the proposed methods, linear dynamic range of 0.1–10 µg m/L with high determination coefficient of R 2 > 0.94 was achieved in this work. Moreover, the obtained limit of detection and recovery values were found to be in reasonable range of 0.03–0.1 µg m/L and 71.7–101.44% (RSDs, 1.2–6.1%), respectively. Finally, by comparing two methods, the LLME was found to be green (organic solvent 500 µL), fast, and economic method for determination of vitamin D 3 in the fortified sunflower oil as compared to Sp-LLE-TLC. Thus, the current work proposed a facile, economic, and green method for fast estimation of vitamin D 3 in the fortified sunflower oil.