The effects of PROTEOLYSIS6 (PRT6) gene suppression on the expression patterns of tomato ethylene response factors
The ubiquitin-mediated proteolysis via Arg N-degron branch regulates various plant developmental processes and responds to multiple stresses. Nevertheless, limited information is available on the significance of the pathway during fruit ripening. This study reports that the suppression of PROTEOLYSI...
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Veröffentlicht in: | Journal of plant biochemistry and biotechnology 2022-03, Vol.31 (1), p.115-123 |
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Sprache: | eng |
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Zusammenfassung: | The ubiquitin-mediated proteolysis via Arg N-degron branch regulates various plant developmental processes and responds to multiple stresses. Nevertheless, limited information is available on the significance of the pathway during fruit ripening. This study reports that the suppression of
PROTEOLYSIS6
(
PRT6
) E3 ligase in tomato through RNAi altered the gene expression of four Ethylene Response Factors (ERFs) (
SIERF77, SIERF2, JERF1
, and
JERF3
). The transient expression of 35S::PRT6_RNAi and PG::PRT6_RNAi constructs successfully down-regulated the
PRT6
expression in leaves and mature green fruits through 35S promoter, and in breaker-stage fruits via polygalacturonase (PG) promoter, respectively. Reverse transcription-quantitative PCR (RT-qPCR) analysis showed an increased expression of
SIERF77
,
SIERF2
, and
JERF1
but decreased
JERF3
expression in the leaves and mature green fruits agroinfiltrated with 35S::PRT6_RNAi. Ripening-specific
SIERF2
showed an increased expression in the breaker-stage fruits agroinfiltrated with PG::PRT6_RNAi, supporting the hypothesis that a low expression level of
PRT6
affected the expression patterns of ERFs in specific tissues of tomato. Conversely, the expression of
pectin methylesterase
(
PME
) decreased in the breaker fruits. The four orthologues of tomato ERFs had been found to be the Arg N-degron substrates in Arabidopsis, with physiological functions being regulated at the protein level. This study showed that the suppression of
PRT6
influenced the expression of these four transcription factors and
PME
. |
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ISSN: | 0971-7811 0974-1275 |
DOI: | 10.1007/s13562-021-00662-4 |