Radiosynthesis, In Vitro and In Vivo Evaluation of 99mTc-Silodosin as a Tumor Marker in Mice

To obtain a candidate tumor-imaging agent, Silodosin was labeled with technetium-99m. The 95% radiochemical yield was reached under the following conditions: 100 µg of Silodosin, 100 µg of SnCl 2 ·2H 2 O, 100 µL of buffer solution (pH 4), 80°C, 15 min. The biodistribution of 99m Tc-Silodosin in normal and tumored mice revealed the potential for using the tracer to diagnose the solid tumor. The tumored/nontumored muscle ratio was 3.33 at 60 min post-injection, indicating high affinity of 99m Tc-Silodosin for the solid tumor. The in vitro uptake of the tracer by PC-3 cells revealed the ability of 99m Tc-Silodosin to conjugate with the adrenoceptor found in the prostate.