The effect of coating application using chitosan enzymatic depolymerization on anthracnose disease suppression in mango (Mangifera indica L.) cv. ‘Arumanis’

Mango cv. ’Arumanis’ is more prone to postharvest disease. An alternative anthracnose disease control is necessary to reduce use of synthetic pesticides. The research was aimed to develop bioformulation against Colletotrichum spp. Seventeen mango phyllosphere isolates belonging to Gram-positive (7 isolates) and Gram-negative (10 isolates) bacteria were obtained from Bogor and Pasuruan. The FAM 1 isolate produced the highest chitinase (14.7±1.11 ppm). Chitosan (CS) depolymerized with chitinase (D3K, BK2P of Bogor isolates) and mixed with tripolyphosphate (TPP) at a concentration ratio of 5:1 (v/v) could inhibit the growth of Colletotrichum spp. compared with control under in vitro test, however, the level of inhibition was lower (