Expression and clinical significance of blood basophil CD16 and CD32 in chronic airway inflammatory disease

Objective To investigate the expression and clinical significance of blood basophil CD16 and CD32 in asthmachronic obstructive pulmonary disease overlap (ACO) and chronic obstructive pulmonary disease (COPD). Methods COPD and ACO patients in the Third Affiliated Hospital of Jinzhou Medical University from December 2019 to December 2020 (set as COPD group and ACO group) and recruited healthy volunteers (set as healthy control group) were selected as research objects. General information including gender, age, medical history, age at onset, body mass index (BMI) and smoking history, and peripheral venous blood were collected. The expressions of CD16 and CD32 in basophil granulocyte enriched groups were detected by flow cytometry. The expression level of tumor necrosis factor-α (TNF-α) in plasma was determined by ELISA. Results Compared with healthy control group, the proportion of CD16+CCR3+ cells, CD16+CD123+HLA-DR– cells and CD16+CCR3+CD123+HLA-DR–cells in peripheral blood mononuclear cell population of ACO g