Synergistic effects of combination indole and ciprofloxacin aantibiotic against persistence Klebsiella pneumoniae isolates

The new therapeutic combinations are urgently required against the persistence bacteria such as Klebsiella pneumoniae which has showed new universal threat. The present work aimed to investigate the synergistic effects of combination indole and ciprofloxacin antibiotic against persistence K. pneumonae isolates. Fifty isolates of K. pneumoniae were collected from different clinical specimens and identified using Vitek-2 system and confirmed by using specific primer for rpoB gene from housekeeping genes. Eight of these isolates were persister cells producers. The current study proved the synergistic effects of combination indole and ciprofloxacin antibiotic against persistence K. pneumonae isolates using the standard microdilution method, and the interaction between the indole and the ciprofloxacin was estimated by calculating the fractional inhibitory concentration (FIC index) of the combination through checkerboard assay. The results of the current study showed that indole-ciprofloxacin combination indole operatively eradicate the persister cells and inhibit the biofilm formation of persistence isolates of K. pneumoniae. The current study confirms the effectiveness of indole and ciprofloxacin to show synergistic anti persister cells of K. pneumoniae isolates.